Tissue Dissociation, OptiTDS™ Kit is a mixture of several tissue dissociationenzymes with appropriate ratios supplied with an optimum buffer. Eachtissue dissociation system is customized to be tissue-specific and cell-specificand proven to work effectively with the corresponding PrimaCell™ system tobring you the best results for each type of primary cells.

KitComponent:

Two aliquots of ready-to-use tissue OptiTDS™

Two aliquots of 10 ml OptiTDS™ digestion buffer

Complete User's Manual.

Quantity and Quality Control: The system contains sufficient materials fordissociation of 2 separate tissue aliquots of up to 10~50 g each. Each lot istested for dissociation performance on tissues from Human, mouse and rat, andprovides freshly prepared kits.

Stability/Storage: The reagents contained in this system isstable at the room temperatures for the normal shipping periods, but thepackage should be stored at -20 °C upon arrival. The reconstituted OptiTDS™should be prepared freshly and only stable for 7-10 days at 4 °C. The unusedkit is stable and effective up to 6 months at the -20 °C.

References:

[1] Andrey Mikhailov, Yoshiyuki Sankai. Donationof neural stem cells? Post mortal viability of spinal cord neuronal cells. AnnuInt Conf IEEE Eng Med Biol Soc. 2018 Jul;2018:5333-5337.

[2] Kazutomo BABA*, Andrey MIKHAILOV** andYoshiyuki SANKAI. Combined automated culture system for tubular structure assemblyand maturation for vascular tissue engineering. Journal of BiomechanicalScience and Engineering. Vol.13, No.3, 2018