Synonyms      OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor. 

Description      Leptin Binding Domain Chicken Recombinant also called Leptin Receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Chicken Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 420-626 of chicken leptin receptor.

The Leptin Binding Domain is purified by proprietary chromatographic techniques. 

Source      Escherichia Coli. 

Physical Appearance       Sterile Filtered colorless solution at a concentration of 0.4 mg/ml. 

Formulation     The protein was filter sterilized and stored at 4°C (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl. 

Stability       Sterile solutions at 0.5mg/ml or less are stable at 4°C for several months. 

Purity       Greater than 99.0% as determined by:

(a) Analysis by SEC-HPLC.

(b) Analysis by SDS-PAGE. 

Amino acid sequence      The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile Biological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio. 

Protein content      Protein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 

2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard. 

Usage      CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.