Synonyms EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1, Deoxyribonuclease-1.
Introduction Deoxyribonuclease I Bovine (bDNase), an enzyme which selectively cleaves DNA. Bovine Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2 .
Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.
Source Extracted from Pancreas.
Physical Appearance Sterile lyophilized freezed dried powder.
Stability 2 years when stored at 4°C, three weeks at 15°C, pH-6.3.
Specific Activity 316IU/1mg.
Unit Defenition One unit will produce a A260 of 0.001/min/mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.