Introduction  Japanese encephalitis previously known as Japanese B encephalitis is a virus from the family Flaviviridae. it is closely related to the West Nile virus and St. Louis encephalitis virus. Positive sense single stranded RNA genome is packaged in the capsid, formed by the capsid protein. The outer envelope is formed by envelope (E) protein and is the protective antigen. It aids in entry of the virus to the inside of the cell. The genome also encodes several nonstructural proteins also (NS1,NS2a,NS2b,NS3,N4a,NS4b,NS5). NS1 is produced as secretory form also. NS3 is a putative helicase, and NS5 is the viral polymerase. It has been noted that the Japanese encephalitis virus (JEV) infects the lumen of the endoplasmic reticulum (ER) and rapidly accumulates substantial amounts of viral proteins for the JEV. Japanese Encephalitis is diagnosed by detection of antibodies in serum and CSF (cerebrospinal fluid) by IgM capture ELISA. 

Description  The E.coli derived recombinant protein contains full length Japanese Encephalitis virus ENV antigen having an Mw of 50kDa. The protein is fused to a 6 histidines tag. 

Purification Method Encephalitis protein was Purified by proprietary chromatographic technique. 

Purity  Encephalitis protein is >90% pure as determined by 10% PAGE (coomassie staining). 

Formulation  20mM Tris-HCl pH 7.2, 8M urea, 200mM NaCl. 

Storage  Encephalitis protein although stable at 4°C for 1 week, should be stored below -18°C.

Please prevent freeze thaw cycles. 

Specificity  Immunoreactive with sera of encephalitis virus infected individuals. 

Applications  Encephalitis antigen is suitable for ELISA and Western blots, excellent antigen for detection of JEV with minimal specificity problems.