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Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells

The Human Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells is for rapid (7-10 days) isolation and growth of normal kidney epithelial cells from human kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Kidney PrimaCell™ 2 System (Cat No. 2-96075):
Human Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells Protocols
Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells
Human Kidney OptiTDS™ 2: Tissue Dissociation System
Human Kidney FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Human Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells Growth Medium
Human Kidney PrimaCell™ 2: Normal Kidney Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Sharpe CC, Dockrell ME. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts. Methods Mol Biol. 2012;806:175-85.
[2] Valente MJ, Henrique R, Vilas-Boas V, Silva R, Bastos Mde L, Carvalho F, Guedes de Pinho P, Carvalho M. Cocaine-induced kidney toxicity: an in vitro study using primary cultured human proximal tubular epithelial cells. Arch Toxicol. 2012 Feb;86(2):249-61. Epub 2011 Oct 8.
[3] Domhan S, Ma L, Tai A, Anaya Z, Beheshti A, Zeier M, Hlatky L, Abdollahi A. Intercellular communication by exchange of cytoplasmic material via tunneling nano-tube like structures in primary human renal epithelial cells. PLoS One. 2011;6(6):e21283. Epub 2011 Jun 27.

Cat No. Description Unit Price Order
2-96075 Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells Kit $ 1132.11 
4-28075 Human Kidney OptiTDS™2: Tissue Dissociation System Kit $ 274.05 
7-15069 Human Kidney FibrOut™2: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 302.40 
9-46075 Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells Growth Medium 5 x 100 ml $ 151.20 
9-47075 Human Kidney PrimaCell™2: Normal Kidney Epithelial Cells Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80075 Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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