The Human Testis PrimaCell™: Normal Sertoli Cells is for rapid (7-10 days) isolation and growth of normal testis sertoli cells from human testis tissues. This system features an optimal condition of tissue dissociation system, Testis OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Testis PrimaCell™ System (Cat No. 2-96105):
Human Testis PrimaCell™: Normal Sertoli Cells Protocols
Human Testis Tissue Preparation Buffer: Normal Sertoli Cells
Human Testis OptiTDS™: Tissue Dissociation System
Human Testis FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Testis PrimaCell™: Normal Sertoli Cells Growth Medium
Human Testis PrimaCell™: Normal Sertoli Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Alves MG, Socorro S, Silva J, Barros A, Sousa M, Cavaco JE, Oliveira PF. In vitro cultured human Sertoli cells secrete high amounts of acetate that is stimulated by 17β-estradiol and suppressed by insulin deprivation. Biochim Biophys Acta. 2012 Jun 12;1823(8):1389-1394.
[2] Musnier A, León K, Morales J, Reiter E, Boulo T, Costache V, Vourc'h P, Heitzler D, Oulhen N, Poupon A, Boulben S, Cormier P, Crépieux P. mRNA-selective translation induced by FSH in primary Sertoli cells. Mol Endocrinol. 2012 Apr;26(4):669-80. Epub 2012 Mar 1.
[3] Oliveira PF, Alves MG, Rato L, Laurentino S, Silva J, Sá R, Barros A, Sousa M, Carvalho RA, Cavaco JE, Socorro S. Effect of insulin deprivation on metabolism and metabolism-associated gene transcript levels of in vitro cultured human Sertoli cells. Biochim Biophys Acta. 2012 Feb;1820(2):84-9. Epub 2011 Nov 20.