The Mouse Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal aortic Smooth Muscle cells from mouse heart tissues. This system features an optimal condition of tissue dissociation system, Heart OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Heart PrimaCell™ 1 System (Cat No. 2-82045):
Mouse Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells Protocols
Mouse Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells
Mouse Heart OptiTDS™ 1: Tissue Dissociation System
Mouse Heart FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells Growth Medium
Mouse Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Kim EJ, Kim DK, Kim SH, Lee KM, Park HS, Kim SH. Alteration of Ryanodine-receptors in Cultured Rat Aortic Smooth Muscle Cells. Korean J Physiol Pharmacol. 2011 Dec;15(6):431-6. Epub 2011 Dec 27.
[2] Yang B, Gwozdz T, Dutko-Gwozdz J, Bolotina VM. Orai1 and Ca2+-independent phospholipase A2 are required for store-operated Icat-SOC current, Ca2+ entry, and proliferation of primary vascular smooth muscle cells. Am J Physiol Cell Physiol. 2012 Mar;302(5):C748-56. doi: 10.1152/ajpcell.00312.2011. Epub 2011 Nov 16.
[3] Lázaro-Suárez ML, Gómez-Zamudio JH, Delgado-Buenrostro NL, Tanoue A, Tsujimoto G, Villalobos-Molina R. Angiotensin II modifies the expression of α(1)-adrenoceptors in aorta smooth muscle cells of α(1D)-adrenoceptor knockout mice. Auton Autacoid Pharmacol. 2011 Jul-Oct;31(3-4):57-63. doi: 10.1111/j.1474-8673.2011.00467.