The Rat Bladder PrimaCell™1: Normal Bladder Epithelial Cells is for rapid (7-10 days) isolation and growth of normal rat bladder epithelial cells from newborn or adult rat bladder tissues. This system features an optimal condition of tissue dissociation system, Bladder OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Bladder PrimaCell™1 System (Cat No. 2-82506):
Rat Bladder PrimaCell™1 Normal Bladder Epithelial Cells Protocols
Rat Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells
Rat Bladder OptiTDS™1: Tissue Dissociation System
Rat Bladder FibrOut™1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Bladder PrimaCell™1: Normal Bladder Epithelial Cells Growth Medium
Rat Bladder PrimaCell™1: Normal Bladder Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Beckel JM, Birder LA. Differential expression and function of nicotinic acetylcholine receptors in the urinary bladder epithelium of the rat. J Physiol. 2012 Mar 15;590(Pt 6):1465-80. Epub 2012 Jan 16.
[2] Chen TC, Tsai JP, Huang HJ, Teng CC, Chien SJ, Kuo HC, Huang WS, Chen CN. Regulation of cyclooxygenase-2 expression in human bladder epithelial cells infected with type I fimbriated uropathogenic E. coli. Cell Microbiol. 2011 Nov;13(11):1703-13. doi: 10.1111/j.1462-5822.2011.01650.x. Epub 2011 Aug 11.