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Rat Colon PrimaCell™2: Normal Colorectal Smooth Muscle Cells

The Rat Colon PrimaCell™ 2:Normal Colorectal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of rat colorectal smooth muscle cells from rat colorectal tumor tissues. This system features an optimal condition of tissue dissociation system, Colon OptiTDS™ that yields 6-8 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Rat Cancer PrimaCell™ 2 System (Cat No. 2-82530):
Rat Cancer PrimaCell™ 2: Normal Colorectal Smooth Muscle Cells Protocols
Rat Cancer Tissue Preparation Buffer 2: Normal Colorectal Smooth Muscle Cells
Rat Cancer OptiTDS™ 2: Tissue Dissociation System
Rat Cancer PrimaCell™ 2:Normal Colorectal Smooth Muscle Cells Growth Medium
Rat Cancer PrimaCell™ 2:Normal Colorectal Smooth Muscle Cells Growth Supplements with Serum (for 600 ml medium)

References:

[1] Kondo J, Endo H, Okuyama H, Ishikawa O, Iishi H, Tsujii M, Ohue M, Inoue M. Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer. Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6235-40. Epub 2011 Mar 28.
[2] Desitter I, Guerrouahen BS, Benali-Furet N, Wechsler J, Jänne PA, Kuang Y, Yanagita M, Wang L, Berkowitz JA, Distel RJ, Cayre YE. A new device for rapid isolation by size and characterization of rare circulating tumor cells. Anticancer Res. 2011 Feb;31(2):427-41.
[3] Brouquet A, Taleb P, Lot AS, Beauchet A, Julie C, Prevost G, Nordlinger B, Penna C. A model of primary culture of colorectal cancer and liver metastasis to predict chemosensitivity. J Surg Res. 2011 Apr;166(2):247-54. Epub 2009 May 26.

Cat No. Description Unit Price Order
2-82530 Rat Colon PrimaCell™2: Normal Colorectal Smooth Muscle Cells Kit $ 1039.50 
4-28237 Rat Colon OptiTDS™2: Tissue Dissociation System Kit $ 255.15 
7-15213 Rat Colon FibrOut™2: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 283.50 
9-25030 Rat Colon PrimaCell™2: Normal Colorectal Smooth Muscle Cells Growth Medium 5 x 100 ml $ 122.85 
9-26030 Rat Colon PrimaCell™2: Normal Colorectal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) set $ 283.50 
9-80237 Rat Colon Tissue Preparation Buffer 2: Normal Colorectal Smooth Muscle Cells 1 x 100 ml $ 132.30 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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