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PrimaCell™ Kit Components:
 (1)  OptiTDS™ (tissue-specific tissue dissociation systems), is a mixture of several tissue dissociation enzymes with appropriate distribution ratios resolved in an optimal buffer.  Each tissue dissociation system is customized to be tissue-specific, cell-specific, and proven to work effectively in accordance with the corresponding PrimaCell™ system to bring the best results for each type of the primary cultured cells.  Each tissue dissociation system is supplied with the optimal working buffers.   

(2)  FibrOut™ (Tissue-specific fibroblast growth inhibitory cocktails), is a complete system that consists of several biochemical compounds and reagents that prevent fibroblast or non-target cell overgrowth.  This ensures that the target cells are allowed to grow at optimal concentrations producing nearly pure cell populations.  Each FibrOut™ is customized to be tissue-specific and cell-specific, and proven to work effectively in accordance with the corresponding PrimaCell™ system to ensure optimal growth and proliferation of the target cells.  Each FibrOut™ is supplied with optimal working buffers.

(3) Basal culture and growth media: Specially formulated medium for target cells cultured and grown at optimized conditions.  Each set of culture and growth media is customized to be tissue-specific, cell-specific, and proven to work efficiently with the corresponding PrimaCell™ system.

(4) Complete growth media Supplements (growth factors and cytokines): Quality controlled batches of growth factors, cytokines and other growth supplements that compose conditioned growth medium for optimal culture of the target cells.  Each set of growth media supplements is customized to be tissue-specific, cell-specific, and proven to work effectively with the corresponding PrimaCell™ system.

(5) Highly purified and specially treated serum or plant additives: Quality controlled batches of animal, human serum, and/or plant additives required in the conditioned growth medium.  Each set of purified and specially treated serum or plant additives is customized to be tissue-specific, cell-specific, and proven to be working effectively in with the corresponding PrimaCell™ system to bring the best results for each type of primary cells.

(6) Buffers for tissue preparation: Sterilized and purified buffers and solutions for treating and preparing tissues prior to the tissue dissociation processes. Each set of buffers for tissue preparation is customized to be tissue-specific and cell-specific, and proven working effectively in accordance with the corresponding PrimaCell™ system.

(7) Detailed instruction manual: Tissue-specific and cell-specific protocols provide standardized laboratory-proven procedures and conditions with step-by-step guidance for the easy isolation and culture of primary cells from tissues. 

 

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