Synonyms    DPP-IV, DPPIV, DPP4, DPP-4, Dipeptidyl Peptidase-IV, Dipeptidyl Peptidase-4, CD26, adenosine deaminase complexing protein 2, Dipeptidylpeptidase-IV, CD-26, ADABP, ADCP2, DPIV, TP103, DP-IV, DP-4, DP4. 

Introduction    Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentrationdependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense. 

Description    Dipeptidyl Peptidase-IV was purified from human placenta cells. 

Source    Human Placenta. 

Physical Appearance    Filtered lyophilized (freeze-dried) powder. 

Formulation    Each 70ng were lyophilized from 29.1?l containing 2mM Tris-HCl, pH 8.0. 

Solubility    Add deionized water to prepare a working stock solution of approximately 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. 

Stability  Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. 

Biological Activity    One unit is defined as the amount of enzyme which will hydrolyze 1μm of H-Gly-Pro-pNA per 1min at 25°C, pH 7.8. 

Specific Activity    13U/mg. 

Purity    Greater than 95% as determined by SDS-PAGE. 

Specifications    The protein was prepared from tissue shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1and 2by FDA required tests. This product is intended for research useonly.