Synonyms DsbC, Thiol:disulfide interchange protein dsbC.
Introduction Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones.
Description Disulfide-Bond Isomerase Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 216 amino acids (21-236) and having a molecular mass of 23.5 kDa.
DsbC is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile filtered colorless solution.
Formulation 1 mg/ml solution containing 20mM phosphate buffer pH 7.5 and 20mM EDTA.
Amino Acid Sequence DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT DDGKHIIQGP MYDVSGTAPVNVTNKMLLKQ LNALEKEMIV YKAPQEKHVI VFTDITCGY CHKLHEQMAD YNALGITVRYLAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YVLGVQLGVSGTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK.
Stability Disulfide-Bond Isomerase Recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Applications 1.Western blot.
2. ELISA.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.