Synonyms DNA polymerase I thermostable, EC 2.7.7.7, Taq polymerase 1.
Description Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74℃ and exhibits a half-life of 40 minutes at 95℃. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses a 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Source Recombinant e.coli contains Thermus aquaticus polymerase gene.
Formulation Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
Unit Definition One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74℃. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25℃), 50mM NaCl, 5mM MgCl2, 200μm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10?g activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. 10×Reaction Buffer with MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.
10×Reaction Buffer without MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100.
Include : 10×Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.
Features Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction ConditionsChoose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10×Reaction Buffer containing 15mM MgCl2. Rely on a Performance-Tested EnzymeOur PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.
Applications PCR, 3´ A-tailing of blunt ends, compatible with Vectors.
Storage Conditions Stable for 5 days at 10℃, for longer period of time store at -20℃.
Storage Buffer Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme.
50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.
Quality Control Tests PCR, activity, SDS-PAGE/purity, endonuclease/nickase.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.