Synonyms Uridine phosphorylase, EC 2.4.2.3, UrdPase, UPase, StUP.
Introduction Uridine phosphorylase from Salmonella typhimurium (StUP) catalyzes the reversible phosphorolysis of uridine with the formation of ribose-1-phosphate and uracil.
Description Uridine phosphorylase Salmonella typhimurium Recombinantproduced in E.Coli is a non-glycosylated, polypeptide having a total molecular mass of 163068 Dalton.
Source Escherichia Coli.
Physical Appearance Sterile Filtered white lyophilized powder.
Formulation The UPase was lyophilized from 1mg/ml solution containing 25mM Tris-HCl, pH 8.0, 0.15M NaCl.
Solubility It is recommended to reconstitute the lyophilized UPase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized UPase although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution UPase should be stored at 4℃ between 2-7 days and for future use below -18℃.
For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by SDS-PAGE.
Activity Determination One unit phosphorylates 1μm of uridine within 1 min at pH 7.3.
Enzyme Activity 30 U/mg protein.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.