Synonyms Thrombin-like enzyme batroxobin, EC 3.4.21.74, BX, Bothrops atrox serine proteinase, Venombin-A, Defibrase, Reptilase, Batroxobin.
Introduction Batroxobin is a serin protease that reduces fibronogen levels and is originally extracted from snake venom of Bothrops Atrox. Batroxobin is used in defibrinogenation and thrombolysis and also has an effect on c-fos gene and growth factor.
Batroxobin can efficiently restrain proliferation of VSMCs, by blocking the release and uptake of Ca2+, thus influencing [Ca2+]i.
Batroxobin is a single chain glycopeptide with a molecular mass of 43kDa on SDS-PAGE gel and its pI-6.6. Batroxobin converts fibrinogen to fibrin through the restricted release of fibrinopeptide-A from fibrinogen to promote blood to clot. Unlike thrombin, it is not affected by heparin and hirudin.
Description The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosilated polypeptide chain containing 231 amino acids and having an Mw of approximately 28-33 kDa.
Source Pichia Pastoris.
Physical Appearance Sterile Filtered white lyophilized powder.
Formulation The Batroxobin protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM sodium acetate buffer, pH 7.4.
Solubility It is recommended to reconstitute the lyophilized Batroxobin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Batroxobin although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 97.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity Batroxobin’s biological activity was found to be of no less than 500KU/mg. (Klobusitzky Unit).
Amino Acid Sequence VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA
EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P.
References Title:Impaired clot retraction in factor XIII A subunit-deficient mice Supplemental materials for: Kasahara et al
Publication:Published online before print December 8, 2009, doi: 10.1182/blood-2009-06-227645 Blood December 8, 2009
Link:http://bloodjournal.hematologylibrary.org/content/115/6/1277/suppl/DC1
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.