Synonyms Thyroglobulin, TGN, AITD3, TG. 

Introduction  Thyroglobulin (TG) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. TG is a large globular dimeric glycoprotein with a total molecular weight of 660 kDa, which occupies a key precursor role in the biosynthesis of the thyroid hormones. Approximately 75% of the total protein content of the thyroid follicle consists of TG. The thyroid gland uses the Thyroglobulin in order to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is produced by the thyroid epithelial cells (thyrocytes) which form spherical follicles. Thyroglobulin is subsequently secreted and stored in the follicular lumen.

Patients with Hashimoto's thyroiditis or Graves' disease, frequently develop antibodies against Thyroglobulin. Tg-specific antibodies help in the diagnosis of the above diseases, however they also may be present in apparently healthy euthyroid individuals. Blood Thyroglobulin levels can be used as a tumor marker for certain kinds of thyroid cancer, and the may also be elevated in cases of Graves' disease. 

Description  Human Thyroglobulin is a glycosylated, polypeptide chain having a total molecular mass of 662 kDa (331 kDa per subunit).

Source Native, Isolated from human thyroid glands. 

Formulation  Human Thyroglobulin is supplied at a concentration of 0.82mg/ml in 16mM HEPES buffer pH-7.6, 240mM sodium chloride, and 20% glycerol.

Storage Conditions  Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°Cfor longer periods of time.

Avoid multiple freeze-thaw cycles. 

Immunological functions 1. Binds to IgG-type human auto-antibodies.

2. Auto-antibodies to thyroglobulin recognize conformation-dependent epitopes.

3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including 1st international reference serum NIBSC 65/93). 

Coating concentration  0.7-1 µg/ml (depending on the type of ELISA plate and coating buffer).

Suitable for biotinylation and iodination. 

Purity Greater than 95% as determined by SDS-PAGE. 

Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.