Introduction  Parainfluenza virus, PIV, contains a group of four distinct serotypes of single-stranded RNA viruses belonging to the paramyxovirus family and two subtypes (4a and 4b). Parainfluenza viruse is a negative-sense, single-stranded RNA viruse that possess fusion and hemagglutinin-neuraminidase glycoprotein "spikes" on their surface. The virion varies in size (average diameter between 150 and 300 nm) and shape. 

Description  MA 104 cells inoculated with parainfluenza 2 virus, strain II ALTB cc 2056. The Parainfluenza-2 Virus was grown on monolayer of MA cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low speed centrifugation (5000 rpm, 40 min). Virus was pelleted by high speed centrifugation at 18000 rpm for 2.5 hours in the SW27 rotor for 20 min at + 4 oC, then the supernatant was collected and protein concentration was determined by Lowry method. 

Inactivation  Thimerosal and beta propiolactone treatment

This product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work. 

Physical Appearance  Sterile Filtered colorless solution FormulationThe Parainfluenza type-2 solution contains 0.1M NaCl, 10mM Tris Hcl, 1mM EDTA pH-8, and 0.1 % sodium azide (NaN3). 

Stability Parainfluenza type-2 although stable 4°C for 4 weeks, should be stored desiccated below -18°C.

Please prevent freeze-thaw cycles. 

Immunological Activity  Serological studies of Parainfluenza virus, immunogen for antibody production. 

Purity  Greater than 90.0% as determined byAnalysis by SDS-PAGE. 

Usage  CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.