Introduction  West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDaprotein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins. 

Description  The E.Coli derived recombinant protein contains the West-Nile N-terminus Envelope Virus immunodominant regions. The protein is fused with 6xHis tag. Mw~42kDa. 

Method  Purified by proprietary chromatographic technique. 

Purity  Protein is >95% pure as determined by 10% PAGE (coomassie staining). 

Formulation  (1mg/ml) 20mM Phosphate buffer pH 7.5. 

Storage  WNV Envelope although stable at 4°C for 1 week, should be stored below -18°C.

Please prevent freeze thaw cycles. 

Specificity  Immunoreactive with sera of West Nile virus infected individuals. 

Applications  Antigen in ELISA and Western blots, excellent antigen for detection of West-Nile virus with minimal specificity problems. 

Amino Acid Sequence  MQLKGTTYGV CSKAFKFLGT PADTGHGTVV LELQYTGTDG PCKVPISSVA SLNDLTPVGR LVTVNPFVSV ATANAKVLIE LEPPFGDSYI VVGRGEQQIN HHWHKSGSSI GKAFTTTLKG ALEMQLKGTT YGVCSKAFKF LGTPADTGHG TVVLELQYTG TDGPCKVPIS SVASLNDLTP VGRLVTVNPFV SVATANAKVL IELEPPFGDS YIVVGRGEQQI NHHWHKSGSS IGKAFTTTLK GALEMQLKGT TYGVCSKAFK FLGTPADTGH GTVVLELQYT GTDGPCKVPI SSVASLNDLT PVGRLVTVNP FVSVATANAK VLIELEPPFG DSYIVVGRGE QQINHHWHKS GSSIGKAFTT TLKGALEHHH HHH .