This construct containing human ER--α36 cDNA cloned into a expression vector that is custom made on pc6+ backbone with the cytomagalovirus (CMV) promoter. It has been shown high efficiency of transfection in the following cell lines, HEK 293 cells, MCF-7 cells, MDA-MB 231 cells, MDA-MB-436 cells, and HB3396 cells.
Cloning sites: EcoR I and BamH I.
Reference
1. Andersson S, Bishop RW, Russell DW. J Biol Chem. 1989 Sep 25;264(27):16249-55.