The Human Bladder PrimaCell™2: Normal Bladder Fibroblasts is for rapid (7-10 days) isolation and growth of normal human bladder fibroblasts from newborn or adult human bladder tissues. This system features an optimal condition of tissue dissociation system, Bladder OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Human Bladder PrimaCell™2 System (Cat No. 2-92008):
Human Bladder PrimaCell™2 Normal Bladder Fibroblasts Protocols
Human Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts
Human Bladder OptiTDS™2: Tissue Dissociation System
Human Bladder PrimaCell™2: Normal Bladder Fibroblasts Growth Medium
Human Bladder PrimaCell™2: Normal Bladder Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Akbal C, Lee SD, Jung C, Rink R, Kaefer M. Upregulation of both PDGF-BB and PDGF-BB receptor in human bladder fibroblasts in response to physiologic hydrostatic pressure. J Pediatr Urol. 2006 Oct;2(5):402-8. Epub 2006 Aug 7.
[2] Drake MJ, Fry CH, Eyden B. Structural characterization of myofibroblasts in the bladder. BJU Int. 2006 Jan;97(1):29-32.
[3] Liang G, Gonzales FA, Jones PA, Orntoft TF, Thykjaer T. Analysis of gene induction in human fibroblasts and bladder cancer cells exposed to the methylation inhibitor 5-aza-2'-deoxycytidine. Cancer Res. 2002 Feb 15;62(4):961-6.