The Human Bladder PrimaCell™3: Normal Bladder Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal human bladder smooth muscle cells from newborn or adult human bladder tissues. This system features an optimal condition of tissue dissociation system, Bladder OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Bladder PrimaCell™3 System (Cat No. 2-96009):
Human Bladder PrimaCell™3 Normal Bladder Smooth Muscle Cells Protocols
Human Bladder Tissue Preparation Buffer 3: Normal Bladder Smooth Muscle Cells
Human Bladder OptiTDS™3: Tissue Dissociation System
Mouse Bladder FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Bladder PrimaCell™3: Normal Bladder Smooth Muscle Cells Growth Medium
Human Bladder PrimaCell™3: Normal Bladder Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Ahvaz HH, Soleimani M, Mobasheri H, Bakhshandeh B, Shakhssalim N, Soudi S, Hafizi M, Vasei M, Dodel M. Effective combination of hydrostatic pressure and aligned nanofibrous scaffolds on human bladder smooth muscle cells: implication for bladder tissue engineering. J Mater Sci Mater Med. 2012 Jun 7.
[2] Hristov KL, Chen M, Afeli SA, Cheng Q, Rovner ES, Petkov GV. Expression and function of KV2-containing channels in human urinary bladder smooth muscle. Am J Physiol Cell Physiol. 2012 Jun;302(11):C1599-608. Epub 2012 Mar 14.
[3Azadzoi KM, Yalla SV, Siroky MB. Human bladder smooth muscle cell damage in disturbed oxygen tension. Urology. 2011 Oct;78(4):967.e9-15. Epub 2011 Aug 27.