The Human Bone PrimaCell™: Normal Osteoblasts is for rapid (7-10 days) isolation and growth of normal rat osteoblasts from newborn or human bone tissues. This system features an optimal condition of tissue dissociation system, Bone OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Bone PrimaCell™ System (Cat No. 2-96012):
Human Bone PrimaCell™ Normal Osteoblasts Protocols
Human Bone Tissue Preparation Buffer: Normal Osteoblasts
Human Bone OptiTDS™: Tissue Dissociation System
Human Bone FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Bone PrimaCell™: Normal Osteoblasts Growth Medium Human Bone PrimaCell™: Normal Osteoblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Tokuda H, Kato K, Natsume H, Kondo A, Kuroyanagi G, Matsushima-Nishiwaki R, Ito Y, Otsuka T, Kozawa O. Involvement of AMP-activated protein kinase in thrombin-stimulated interleukin-6 synthesis in osteoblasts. J Mol Endocrinol. 2012 May 29.
[2] Hammoudi TM, Rivet CA, Kemp ML, Lu H, Temenoff JS. Three-Dimensional In Vitro Tri-Culture Platform to Investigate Effects of Crosstalk Between Mesenchymal Stem Cells, Osteoblasts, and Adipocytes. Tissue Eng Part A. 2012 May 15.
[3] Bakker AD, Klein-Nulend J. Osteoblast isolation from murine calvaria and long bones. Methods Mol Biol. 2012;816:19-29.