The Human Brain PrimaCell™ 3: Normal Cerebral Artery Vascular Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal human cerebral artery vascular smooth muscle cells from newborn or adult human brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Brain PrimaCell™ 3 System (Cat No. 2-96015):
Human Brain PrimaCell™ 3: Normal Cerebral Artery Vascular Smooth Muscle Cells Protocols
Human Brain Tissue Preparation Buffer 3: Normal Cerebral Artery Vascular Smooth Muscle Cells
Human Brain OptiTDS™ 3: Tissue Dissociation System
Human Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Brain PrimaCell™ 3: Normal Cerebral Artery Vascular Smooth Muscle Cells Growth Medium
Human Brain PrimaCell™ 3: Normal Cerebral Artery Vascular Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Povlsen GK, Waldsee R, Ahnstedt H, Kristiansen KA, Johansen FF, Edvinsson L. In vivo experimental stroke and in vitro organ culture induce similar changes in vasoconstrictor receptors and intracellular calcium handling in rat cerebral arteries. Exp Brain Res. 2012 Jun;219(4):507-20. Epub 2012 May 15.
[2] Sharma N, Cho DH, Kim SY, Bhattarai JP, Hwang PH, Han SK. Magnesium sulfate suppresses L-type calcium currents on the basilar artery smooth muscle cells in rabbits. Neurol Res. 2012 Apr;34(3):291-6. Epub 2012 Mar 20.
[3] Gonzales AL, Earley S. Endogenous cytosolic Ca(2+) buffering is necessary for TRPM4 activity in cerebral artery smooth muscle cells. Cell Calcium. 2012 Jan;51(1):82-93. doi: 10.1016/j.ceca.2011.11.004. Epub 2011 Dec 7.