The Human Brain PrimaCell™ 4: Normal Cerebral Vascular Endothelial Cells is for rapid (7-10 days) isolation and growth of normal human cerebral vascular endothelial cells from newborn or adult human brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Brain PrimaCell™ 4 System (Cat No. 2-96016):
Human Brain PrimaCell™ 4: Normal Cerebral Vascular Endothelial Cells Protocols
Human Brain Tissue Preparation Buffer 4: Normal Cerebral Vascular Endothelial Cells
Human Brain OptiTDS™ 4: Tissue Dissociation System
Human Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Brain PrimaCell™ 4: Normal Cerebral Vascular Endothelial Cells Growth Medium
Human Brain PrimaCell™ 4: Normal Cerebral Vascular Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Tung HH, Lee SL. Neural transmembrane protease and endothelial Gs-protein activation in cell contact-dependent signaling between neural stem/progenitor cells and brain endothelial cells. J Biol Chem. 2012 May 10.
[2] Gauthier SA, Sahoo S, Jung SS, Levy E. Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain. Methods Mol Biol. 2012;849:261-74.
[3] Skinner RA, Gibson RM, Rothwell NJ, Pinteaux E, Penny JI. Transport of interleukin-1 across cerebromicrovascular endothelial cells. Br J Pharmacol. 2009 Apr;156(7):1115-23. Epub 2009 Mar 4.