The Human Brain PrimaCell™ 9: Normal Nerve Microglia is for rapid (7-10 days) isolation and growth of normal human nerve microglia from newborn or adult human brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Brain PrimaCell™ 9 System (Cat No. 2-96021):
Human Brain PrimaCell™ 9: Normal Nerve Microglia Protocols
Human Brain Tissue Preparation Buffer 9: Normal Nerve Microglia
Human Brain OptiTDS™ 9: Tissue Dissociation System
Human Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Brain PrimaCell™ 9: Normal Nerve Microglia Growth Medium
Human Brain PrimaCell™9: Normal Nerve Microglia Growth Supplements with Serum (for 500 ml medium)

References:

[1] Uliasz TF, Hamby ME, Jackman NA, Hewett JA, Hewett SJ. Generation of primary astrocyte cultures devoid of contaminating microglia. Methods Mol Biol. 2012;814:61-79.
[2] Tseng YT, Hsu YY, Shih YT, Lo YC. Paeonol attenuates microglia-mediated inflammation and oxidative stress-induced neurotoxicity in rat primary microglia and cortical neurons. Shock. 2012 Mar;37(3):312-8.
[3] Silva Bastos LF, Pinheiro de Oliveira AC, Magnus Schlachetzki JC, Fiebich BL. Minocycline reduces prostaglandin E synthase expression and 8-isoprostane formation in LPS-activated primary rat microglia. Immunopharmacol Immunotoxicol. 2011 Sep;33(3):576-80. Epub 2011 Jan 12.