The Human Breast PrimaCell™: Normal Mammary Epithelial Cells is for rapid (7-10 days) isolation and growth of normal human mammary epithelial cells from newborn or adult human breast tissues. This system features an optimal condition of tissue dissociation system, Breast OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Human Breast PrimaCell™ System (Cat No. 2-96023):
Human Breast PrimaCell™: Normal Mammary Epithelial Cells Protocols
Human Breast Tissue Preparation Buffer: Normal Mammary Epithelial Cells
Human Breast OptiTDS™: Tissue Dissociation System
Human Breast FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Breast PrimaCell™: Normal Mammary Epithelial Cells Growth Medium
Human Breast PrimaCell™: Normal Mammary Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Miao J, Fa Y, Gu B, Zhu W, Zou S. Taurine attenuates lipopolysaccharide-induced disfunction in mouse mammary epithelial cells. Cytokine. 2012 Jul;59(1):35-40. Epub 2012 May 10.
[2] Fedrowitz M, Hass R, Bertram C, Loscher W. Salivary alpha-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells. J Exp Clin Cancer Res. 2011 Oct 25;30(1):102.
[3] Bouchet BP, Puisieux A, Galmarini CM. βIII-Tubulin is required for interphase microtubule dynamics in untransformed human mammary epithelial cells. Eur J Cell Biol. 2011 Oct;90(10):872-8. Epub 2011 Aug 4.