The Human Cancer PrimaCell™ 18: Uterine Tumor Cells is for rapid (7-10 days) isolation and growth of human uterine tumor cells from human uterine tumor tissues. This system features an optimal condition of tissue dissociation system, Cancer OptiTDS™ that yields 6-8 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.
Human Cancer PrimaCell™ 18 System (Cat No. 2-96042):
Human Cancer PrimaCell™ 18: Uterine Tumor Cells Protocols
Human Cancer Tissue Preparation Buffer 18: Uterine Tumor Cells
Human Cancer OptiTDS™ 18: Tissue Dissociation System
Human Cancer PrimaCell™ 18: Uterine Tumor Cells Growth Medium
Human Cancer PrimaCell™ 18: Uterine Tumor Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Fotheringham S, Levanon K, Drapkin R. Ex vivo culture of primary human fallopian tube epithelial cells. J Vis Exp. 2011 May 9;(51). pii: 2728. doi: 10.3791/2728.
[2] A novel three-dimensional culture system of polarized epithelial cells to study endometrial carcinogenesis. Eritja N, Llobet D, Domingo M, Santacana M, Yeramian A, Matias-Guiu X, Dolcet X. Am J Pathol. 2010 Jun;176(6):2722-31. Epub 2010 Apr 15.
[3] Characterization and phenotypic variation with passage number of cultured human endometrial adenocarcinoma cells. Kato S, Espinoza N, Lange S, Villalón M, Cuello M, Owen GI. Tissue Cell. 2008 Apr;40(2):95-102. Epub 2007 Nov 26.