The Human Cartilage PrimaCell™ : Normal Articular Cartilage is for rapid (7-10 days) isolation and growth of normal articular cartilage from newborn or adult human cartilage tissues. This system features an optimal condition of tissue dissociation system, Cartilage OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Human Cartilage PrimaCell™ System (Cat No. 2-96043):
Human Cartilage PrimaCell™ Normal Articular Cartilage Protocols
Human Cartilage Tissue Preparation Buffer : Normal Articular Cartilage
Human Cartilage OptiTDS™ : Tissue Dissociation System
Human Cartilage FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Cartilage PrimaCell™ : Normal Articular Cartilage Growth Medium
Human Cartilage PrimaCell™ : Normal Articular Cartilage Growth Supplements with Serum (for 500 ml medium)
References:
[1] Im HJ, Li X, Chen D, Yan D, Kim J, Ellman MB, Stein GS, Cole B, Kc R, Cs-Szabo G, van Wijnen AJ. Biological effects of the plant-derived polyphenol resveratrol in human articular cartilage and chondrosarcoma cells. J Cell Physiol. 2012 Jan 17. doi: 10.1002/jcp.24049.
[2] Chang HX, Yang L, Li Z, Chen G, Dai G. Age-related biological characterization of mesenchymal progenitor cells in human articular cartilage. Orthopedics. 2011 Aug 8;34(8):e382-8. doi: 10.3928/01477447-20110627-06.
[3] Seol JW, Lee HB, Lee YJ, Lee YH, Kang HS, Kim IS, Kim NS, Park SY. Hypoxic resistance to articular chondrocyte apoptosis--a possible mechanism of maintaining homeostasis of normal articular cartilage. FEBS J. 2009 Dec;276(24):7375-85. Epub .