The Human Cervix PrimaCell™ 1: Normal Cervical Epithelial Cells is for rapid (7-10 days) isolation and growth of normal cervical epithelial cells from human cervix tissues. This system features an optimal condition of tissue dissociation system, Cervix OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Human Cervix PrimaCell™ 1 System (Cat No. 2-96044):
Human Cervix PrimaCell™ 1 Normal Cervical Epithelial Cells Protocols
Human Cervix Tissue Preparation Buffer 1: Normal Cervical Epithelial Cells
Human Cervix OptiTDS™ 1: Tissue Dissociation System
Human Cervix FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Cervix PrimaCell™ 1: Normal Cervical Epithelial Cells Growth Medium
Human Cervix PrimaCell™ 1: Normal Cervical Epithelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Jennings MP, Jen FE, Roddam LF, Apicella MA, Edwards JL. Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells. Cell Microbiol. 2011 Jun;13(6):885-96. doi: 10.1111/j.1462-5822.2011.01586.x. Epub 2011 Mar 4.
[2] Vats V, Agrawal T, Salhan S, Mittal A. Characterization of apoptotic activities during chlamydia trachomatis infection in primary cervical epithelial cells. Immunol Invest. 2010;39(7):674-87.
[3] Edwards JL. Neisseria gonorrhoeae survival during primary human cervical epithelial cell infection requires nitric oxide and is augmented by progesterone. Infect Immun. 2010 Mar;78(3):1202-13. Epub 2010 Jan 4.