The Human Endothelium PrimaCell™: Normal Vascular Endothelial Cells is for rapid (7-10 days) isolation and growth of normal vascular endothelial cells from human endothelial tissues. This system features an optimal condition of tissue dissociation system, Endothelium OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Endothelium PrimaCell™ System (Cat No. 2-96054):
Human Endothelium PrimaCell™: Normal Vascular Endothelial Cells Protocols
Human Endothelium Tissue Preparation Buffer: Normal Endothelium Endothelial Cells
Human Endothelium OptiTDS™: Tissue Dissociation System
Human Endothelium FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Endothelium PrimaCell™: Normal Vascular Endothelial Cells Growth Medium
Human Endothelium PrimaCell™: Normal Vascular Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Sullivan MN, Francis M, Pitts NL, Taylor MS, Earley S. Optical Recording Reveals Novel Properties of GSK1016790A-Induced TRPV4 Channel Activity in Primary Human Endothelial Cells. Mol Pharmacol. 2012 Jun 11.
[2] Olafsson IH, Vilhjalmsson DT, Thormodsson FR. Preparation of cultured human vascular cells. Methods Mol Biol. 2012;849:245-59.
[3] Nolte A, Hossfeld S, Schroeppel B, Mueller A, Stoll D, Walker T, Wendel HP, Krastev R. Impact of polyelectrolytes and their corresponding multilayers to human primary endothelial cells. J Biomater Appl. 2012 Mar 28.