The Human Esophagus PrimaCell™ 2: Normal Esophageal Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal esophageal smooth muscle cells from human esophagus tissues. This system features an optimal condition of tissue dissociation system, Esophagus OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Esophagus PrimaCell™ 2 System (Cat No. 2-96056):
Human Esophagus PrimaCell™ 2: Normal Esophageal Smooth Muscle Cells Protocols
Human Esophagus Tissue Preparation Buffer 2: Normal Esophagus Smooth Muscle Cells
Human Esophagus OptiTDS™ 2: Tissue Dissociation System
Human Esophagus FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Human Esophagus PrimaCell™ 2: Normal Esophageal Smooth Muscle Cells Growth Medium
Human Esophagus PrimaCell™ 2: Normal Esophageal Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

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[2] Aceves SS, Chen D, Newbury RO, Dohil R, Bastian JF, Broide DH. Mast cells infiltrate the esophageal smooth muscle in patients with eosinophilic esophagitis, express TGF-β1, and increase esophageal smooth muscle contraction. J Allergy Clin Immunol. 2010 Dec;126(6):1198-204.e4. Epub 2010 Nov 3.
[3] Zhu Y, Chan-Park MB. Density quantification of collagen grafted on biodegradable polyester: its application to esophageal smooth muscle cell. Anal Biochem. 2007 Apr 1;363(1):119-27. Epub 2007 Jan 12.