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Human Eye PrimaCell™2: Normal Corneal Epithelial Cells

The Human Eye PrimaCell™ 2: Normal Corneal Epithelial Cells is for rapid (7-10 days) isolation and growth of normal corneal epithelial cells from human eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Eye PrimaCell™ 2 System (Cat No. 2-96058):
Human Eye PrimaCell™ 2: Normal Corneal Epithelial Cells Protocols
Human Eye Tissue Preparation Buffer 2: Normal Corneal Epithelial Cells
Human Eye OptiTDS™ 2: Tissue Dissociation System
Human Eye FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Human Eye PrimaCell™ 2: Normal Corneal Epithelial Cells Growth Medium
Human Eye PrimaCell™ 2: Normal Corneal Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Kim SW, Seo KY, Rhim T, Kim EK. Effect of retinoic acid on epithelial differentiation and mucin expression in primary human corneal limbal epithelial cells. Curr Eye Res. 2012 Jan;37(1):33-42. Epub 2011 Oct 26. Erratum in: Curr Eye Res. 2012 Jan;37(1):42.
[2] Oliveira LA, Kim C, Sousa LB, Schwab IR, Rosenblatt MI. Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector. Arq Bras Oftalmol. 2010 Sep-Oct;73(5):447-53.
[3] Seaman CW, Whittingham A, Guest R, Warren N, Olson MJ, Guerriero FJ, Adriaens E, De Wever B. An evaluation of a cultured human corneal epithelial tissue model for the determination of the ocular irritation potential of pharmaceutical process materials. Toxicol In Vitro. 2010 Sep;24(6):1862-70. Epub 2010 Mar 17.

Cat No. Description Unit Price Order
2-96058 Human Eye PrimaCell™2: Normal Corneal Epithelial Cells Kit $ 1132.11 
4-28058 Human Eye OptiTDS™2: Tissue Dissociation System Kit $ 274.05 
7-15053 Human Eye FibrOut™2: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 302.40 
9-46058 Human Eye PrimaCell™2: Normal Corneal Epithelial Cells Growth Medium 5 x 100 ml $ 151.20 
9-47058 Human Eye PrimaCell™2: Normal Corneal Epithelial Cells Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80058 Human Eye Tissue Preparation Buffer 2: Normal Corneal Epithelial Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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