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Human Eye PrimaCell™5: Normal Retinal Pigment Epithelial Cells

The Human Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells is for rapid (7-10 days) isolation and growth of normal retinal pigment epithelial Cells from human eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Eye PrimaCell™ 5 System (Cat No. 2-96061):
Human Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells Protocols
Human Eye Tissue Preparation Buffer 5: Normal Retinal Pigment Epithelial Cells
Human Eye OptiTDS™ 5: Tissue Dissociation System
Human Eye FibrOut™ 5: Fibroblast Inhibitory System (for 500 ml medium)
Human Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells Growth Medium
Human Eye PrimaCell™ 5: Normal Retinal Pigment Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Bandyopadhyay M, Rohrer B. Matrix metalloproteinase activity creates pro-angiogenic environment in primary human retinal pigment epithelial cells exposed to complement. Invest Ophthalmol Vis Sci. 2012 Apr 18;53(4):1953-61. Print 2012 Apr.
[2] Chung EJ, Chun JN, Jung SA, Cho JW, Lee JH. TGF-β-stimulated aberrant expression of class III β-tubulin via the ERK signaling pathway in cultured retinal pigment epithelial cells. Biochem Biophys Res Commun. 2011 Nov 18;415(2):367-72. Epub 2011 Oct 20.
[3] Yu AL, Lorenz RL, Haritoglou C, Kampik A, Welge-Lussen U. Biological effects of native and oxidized low-density lipoproteins in cultured human retinal pigment epithelial cells. Exp Eye Res. 2009 Mar;88(3):495-503. Epub 2008 Nov 27.

Cat No. Description Unit Price Order
2-96061 Human Eye PrimaCell™5: Normal Retinal Pigment Epithelial Cells Kit $ 1228.50 
4-28061 Human Eye OptiTDS™5: Tissue Dissociation System Kit $ 274.05 
7-15056 Human Eye FibrOut™5: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 302.40 
9-46061 Human Eye PrimaCell™5: Normal Retinal Pigment Epithelial Cells Growth Medium 5 x 100 ml $ 151.20 
9-47061 Human Eye PrimaCell™5: Normal Retinal Pigment Epithelial Cells Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80061 Human Eye Tissue Preparation Buffer 5: Normal Retinal Pigment Epithelial Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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