The Human Fat PrimaCell™ 2: Normal Preadipocytes is for rapid (7-10 days) isolation and growth of normal preadipocytes from human fat tissues. This system features an optimal condition of tissue dissociation system, Fat OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Fat PrimaCell™ 2 System (Cat No. 2-96063):
Human Fat PrimaCell™ 2: Normal Preadipocytes Protocols
Human Fat Tissue Preparation Buffer 2: Normal Preadipocytes
Human Fat OptiTDS™ 2: Tissue Dissociation System
Human Fat FibrOut™ 2: Fibroblast Inhibitory System (for 500 ml medium)
Human Fat PrimaCell™ 2: Normal Preadipocytes Growth Medium
Human Fat PrimaCell™ 2: Normal Preadipocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Hernandez A, de Mena RM, Martin E, Obregon MJ. Differences in the response of UCP1 mRNA to hormonal stimulation between rat and mouse primary cultures of brown adipocytes. Cell Physiol Biochem. 2011;28(5):969-80. Epub 2011 Dec 15.
[2] Sun C, Qi R, Wang L, Yan J, Wang Y. p38 MAPK regulates calcium signal-mediated lipid accumulation through changing VDR expression in primary preadipocytes of mice. Mol Biol Rep. 2012 Mar;39(3):3179-84. Epub 2011 Jun 24.
[3] Hemmrich K, Gummersbach C, Paul NE, Goy D, Suschek CV, Kröncke KD, Pallua N. Nitric oxide and downstream second messenger cGMP and cAMP enhance adipogenesis in primary human preadipocytes. Cytotherapy. 2010 Jul;12(4):547-53.