The Human Fat PrimaCell™ 3: Normal Subcutaneous Fat Cells is for rapid (7-10 days) isolation and growth of normal subcutaneous fat cells from human fat tissues. This system features an optimal condition of tissue dissociation system, Fat OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Fat PrimaCell™ 3 System (Cat No. 2-96064):
Human Fat PrimaCell™ 3: Normal Subcutaneous Fat Cells Protocols
Human Fat Tissue Preparation Buffer 3: Normal Subcutaneous Fat Cells
Human Fat OptiTDS™ 3: Tissue Dissociation System
Human Fat FibrOut™ 3: Fibroblast Inhibitory System (for 500 ml medium)
Human Fat PrimaCell™ 3: Normal Subcutaneous Fat Cells Growth Medium
Human Fat PrimaCell™ 3: Normal Subcutaneous Fat Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Rittig K, Dolderer JH, Balletshofer B, Machann J, Schick F, Meile T, Küper M, Stock UA, Staiger H, Machicao F, Schaller HE, Königsrainer A, Häring HU, Siegel-Axel DI. The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells. Diabetologia. 2012 May;55(5):1514-25. Epub 2012 Feb 13.
[2] Jufvas A, Strålfors P, Vener AV. Histone variants and their post-translational modifications in primary human fat cells. PLoS One. 2011 Jan 7;6(1):e15960.
[3] Bujalska IJ, Durrani OM, Abbott J, Onyimba CU, Khosla P, Moosavi AH, Reuser TT, Stewart PM, Tomlinson JW, Walker EA, Rauz S. Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat. J Endocrinol. 2007 Feb;192(2):279-88.