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Human Heart PrimaCell™1: Normal Aortic Smooth Muscle Cells

The Human Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells is for rapid (7-10 days) isolation and growth of normal aortic Smooth Muscle cells from human heart tissues. This system features an optimal condition of tissue dissociation system, Heart OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Heart PrimaCell™ 1 System (Cat No. 2-96066):
Human Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells Protocols
Human Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells
Human Heart OptiTDS™ 1: Tissue Dissociation System
Human Heart FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Human Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells Growth Medium
Human Heart PrimaCell™ 1: Normal Aortic Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Kim EJ, Kim DK, Kim SH, Lee KM, Park HS, Kim SH. Alteration of Ryanodine-receptors in Cultured Rat Aortic Smooth Muscle Cells. Korean J Physiol Pharmacol. 2011 Dec;15(6):431-6. Epub 2011 Dec 27.
[2] Yang B, Gwozdz T, Dutko-Gwozdz J, Bolotina VM. Orai1 and Ca2+-independent phospholipase A2 are required for store-operated Icat-SOC current, Ca2+ entry, and proliferation of primary vascular smooth muscle cells. Am J Physiol Cell Physiol. 2012 Mar;302(5):C748-56. doi: 10.1152/ajpcell.00312.2011. Epub 2011 Nov 16.
[3] Lázaro-Suárez ML, Gómez-Zamudio JH, Delgado-Buenrostro NL, Tanoue A, Tsujimoto G, Villalobos-Molina R. Angiotensin II modifies the expression of α(1)-adrenoceptors in aorta smooth muscle cells of α(1D)-adrenoceptor knockout mice. Auton Autacoid Pharmacol. 2011 Jul-Oct;31(3-4):57-63. doi: 10.1111/j.1474-8673.2011.00467.

Cat No. Description Unit Price Order
2-96066 Human Heart PrimaCell™1: Normal Aortic Smooth Muscle Cells Kit $ 1228.50 
4-28066 Human Heart OptiTDS™1: Tissue Dissociation System Kit $ 274.05 
7-15061 Human Heart FibrOut™1: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 302.40 
9-46066 Human Heart PrimaCell™1: Normal Aortic Smooth Muscle Cells Growth Medium 5 x 100 ml $ 151.20 
9-47066 Human Heart PrimaCell™1: Normal Aortic Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80066 Human Heart Tissue Preparation Buffer 1: Normal Aortic Smooth Muscle Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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