The Human Heart PrimaCell™ 3: Normal Heart Fibroblasts is for rapid (7-10 days) isolation and growth of normal heart fibroblasts from human heart tissues. This system features an optimal condition of tissue dissociation system, Heart OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Human Heart PrimaCell™ 3 System (Cat No. 2-96068):
Human Heart PrimaCell™ 3: Normal Heart Fibroblasts Protocols
Human Heart Tissue Preparation Buffer 3: Normal Heart Fibroblasts
Human Heart OptiTDS™ 3: Tissue Dissociation System
Human Heart PrimaCell™ 3: Normal Heart Fibroblasts Growth Medium
Human Heart PrimaCell™ 3: Normal Heart Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Golden HB, Gollapudi D, Gerilechaogetu F, Li J, Cristales RJ, Peng X, Dostal DE. Isolation of cardiac myocytes and fibroblasts from neonatal rat pups. Methods Mol Biol. 2012;843:205-14.
[2] Epperson SA, Brunton LL, Ramirez-Sanchez I, Villarreal F. Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts. Am J Physiol Cell Physiol. 2009 May;296(5):C1171-7. Epub 2009 Feb 25.
[3] Venkatachalam K, Mummidi S, Cortez DM, Prabhu SD, Valente AJ, Chandrasekar B. Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts. Am J Physiol Heart Circ Physiol. 2008 May;294(5):H2078-87. Epub 2008 Feb 29.