The Human Kidney PrimaCell™ 3: Normal Renal Artery Endothelial Cells is for rapid (7-10 days) isolation and growth of normal renal artery endothelial cells from human kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Human Kidney PrimaCell™ 3 System (Cat No. 2-96076):
Human Kidney PrimaCell™ 3: Normal Renal Artery Endothelial Cells Protocols
Human Kidney Tissue Preparation Buffer 3: Normal Renal Artery Endothelial Cells
Human Kidney OptiTDS™ 3: Tissue Dissociation System
Human Kidney FibrOut™ 3: Fibroblast Inhibitory System (for 500 ml medium)
Human Kidney PrimaCell™ 3: Normal Renal Artery Endothelial Cells Growth Medium
Human Kidney PrimaCell™ 3: Normal Renal Artery Endothelial Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Thibonnier M, Conarty DM, Preston JA, Plesnicher CL, Dweik RA, Erzurum SC. Human vascular endothelial cells express oxytocin receptors. Endocrinology. 1999 Mar;140(3):1301-9.
[2] Farris AB, Taheri D, Kawai T, Fazlollahi L, Wong W, Tolkoff-Rubin N, Spitzer TR, Iafrate AJ, Preffer FI, Locascio SA, Sprangers B, Saidman S, Smith RN, Cosimi AB, Sykes M, Sachs DH, Colvin RB. Acute renal endothelial injury during marrow recovery in a cohort of combined kidney and bone marrow allografts. Am J Transplant. 2011 Jul;11(7):1464-77. doi: 10.1111/j.1600-6143.2011.03572.x. Epub 2011 Jun 10.
[3] Nowatzki J, de Sene RV, Paludo KS, Veiga SS, Oliver C, Jamur MC, Nader HB, Trindade ES, Franco CR. Brown spider venom toxins interact with cell surface and are endocytosed by rabbit endothelial cells. Toxicon. 2010 Sep 15;56(4):535-43. Epub 2010 Jun 1.