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Human Kidney PrimaCell™4: Normal Renal Firbroblasts

The Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts is for rapid (7-10 days) isolation and growth of normal renal firbroblasts from human kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Human Kidney PrimaCell™ 4 System (Cat No. 2-96077):
Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts Protocols
Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts
Human Kidney OptiTDS™ 4: Tissue Dissociation System
Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts Growth Medium
Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Sharpe CC, Dockrell ME. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts. Methods Mol Biol. 2012;806:175-85.
[2] Nishi H, Inagi R, Kawada N, Yoshizato K, Mimura I, Fujita T, Nangaku M. Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions. Am J Pathol. 2011 Jan;178(1):128-39. Epub 2010 Dec 23.
[3] Mou S, Wang Q, Shi B, Gu L, Ni Z. Hepatocyte growth factor suppresses transforming growth factor-beta-1 and type III collagen in human primary renal fibroblasts. Kaohsiung J Med Sci. 2009 Nov;25(11):577-87.

Cat No. Description Unit Price Order
2-96077 Human Kidney PrimaCell™4: Normal Renal Firbroblasts Kit $ 1228.50 
4-28077 Human Kidney OptiTDS™4: Tissue Dissociation System Kit $ 274.05 
9-46077 Human Kidney PrimaCell™4: Normal Renal Firbroblasts Growth Medium 5 x 100 ml $ 151.20 
9-47077 Human Kidney PrimaCell™4: Normal Renal Firbroblasts Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80077 Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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