The Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts is for rapid (7-10 days) isolation and growth of normal renal firbroblasts from human kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.
Human Kidney PrimaCell™ 4 System (Cat No. 2-96077):
Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts Protocols
Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts
Human Kidney OptiTDS™ 4: Tissue Dissociation System
Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts Growth Medium
Human Kidney PrimaCell™ 4: Normal Renal Firbroblasts Growth Supplements with Serum (for 500 ml medium)
References:
[1] Sharpe CC, Dockrell ME. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts. Methods Mol Biol. 2012;806:175-85.
[2] Nishi H, Inagi R, Kawada N, Yoshizato K, Mimura I, Fujita T, Nangaku M. Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions. Am J Pathol. 2011 Jan;178(1):128-39. Epub 2010 Dec 23.
[3] Mou S, Wang Q, Shi B, Gu L, Ni Z. Hepatocyte growth factor suppresses transforming growth factor-beta-1 and type III collagen in human primary renal fibroblasts. Kaohsiung J Med Sci. 2009 Nov;25(11):577-87.