The Human Kidney PrimaCell™ 5: Normal Renal Mesangial Cells is for rapid (7-10 days) isolation and growth of normal renal mesangial cells from human kidney tissues. This system features an optimal condition of tissue dissociation system, Kidney OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Kidney PrimaCell™ 5 System (Cat No. 2-96078):
Human Kidney PrimaCell™ 5: Normal Renal Mesangial Cells Protocols
Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells
Human Kidney OptiTDS™ 5: Tissue Dissociation System
Human Kidney FibrOut™ 5: Fibroblast Inhibitory System (for 500 ml medium)
Human Kidney PrimaCell™ 5: Normal Renal Mesangial Cells Growth Medium
Human Kidney PrimaCell™ 5: Normal Renal Mesangial Cells Growth Supplements with Serum (for 500 ml medium)

References:

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[2] Lim JC, Lim SK, Park MJ, Kim GY, Han HJ, Park SH. Cannabinoid receptor 1 mediates high glucose-induced apoptosis via endoplasmic reticulum stress in primary cultured rat mesangial cells. Am J Physiol Renal Physiol. 2011 Jul;301(1):F179-88. Epub 2011 Feb 16.
[3] Zhang HM, Zhang Y, Zhang BX. The role of mitochondrial complex III in melatonin-induced ROS production in cultured mesangial cells. J Pineal Res. 2011 Jan;50(1):78-82. doi: 10.1111/j.1600-079X.2010.00815.x. Epub 2010 Oct 20.