The Human Lung PrimaCell™ 3: Normal Pulmonary Artery Fibroblasts is for rapid (7-10 days) isolation and growth of normal pulmonary artery fibroblasts from human lung tissues. This system features an optimal condition of tissue dissociation system, Lung OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Human Lung PrimaCell™ 3 System (Cat No. 2-96083):
Human Lung PrimaCell™ 3: Normal Pulmonary Artery Fibroblasts Protocols
Human Lung Tissue Preparation Buffer 3: Normal Pulmonary Artery Fibroblasts
Human Lung OptiTDS™ 3: Tissue Dissociation System
Human Lung PrimaCell™ 3: Normal Pulmonary Artery Fibroblasts Growth Medium
Human Lung PrimaCell™ 3: Normal Pulmonary Artery Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Nam YH, Lee SK, Sammut D, Davies DE, Howarth PH. Preliminary study of the cellular characteristics of primary bronchial fibroblasts in patients with asthma: expression of alpha-smooth muscle actin, fibronectin containing extra type III domain A, and smoothelin. J Investig Allergol Clin Immunol. 2012;22(1):20-7.
[2] Zhang J, Feng MX, Qu JM. Low dose theophylline showed an inhibitory effect on the production of IL-6 and IL-8 in primary lung fibroblast from patients with COPD. Mediators Inflamm. 2012;2012:492901. Epub 2012 Jan 26.
[3] Van Ly D, King NJ, Moir LM, Burgess JK, Black JL, Oliver BG. Effects of β(2) Agonists, Corticosteroids, and Novel Therapies on Rhinovirus-Induced Cytokine Release and Rhinovirus Replication in Primary Airway Fibroblasts. J Allergy (Cairo). 2011;2011:457169. Epub 2011 Oct 24.