The Human Lymph PrimaCell™ 3: Normal Lymphatic Fibroblasts is for rapid (7-10 days) isolation and growth of normal lymphatic fibroblasts from human lymph tissues. This system features an optimal condition of tissue dissociation system, Lymph OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Human Lymph PrimaCell™ 3 System (Cat No. 2-96089):
Human Lymph PrimaCell™ 3: Normal Lymphatic Fibroblasts Protocols
Human Lymph Tissue Preparation Buffer 3: Normal Lymphatic Fibroblasts
Human Lymph OptiTDS™ 3: Tissue Dissociation System
Human Lymph PrimaCell™ 3: Normal Lymphatic Fibroblasts Growth Medium
Human Lymph PrimaCell™ 3: Normal Lymphatic Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Neri S, Ishii G, Taira T, Hishida T, Yoshida J, Nishimura M, Nagai K, Ochiai A. Recruitment of Podoplanin Positive Cancer-Associated Fibroblasts in Metastatic Lymph Nodes Predicts Poor Prognosis in Pathological N2 Stage III Lung Adenocarcinoma. Ann Surg Oncol. 2012 Jun 6.
[2] Chaiwun B, Sukhamwang N, Trakultivakorn H, Saha B, Young L, Tsao-Wei D, Naritoku WY, Groshen S, Taylor CR, Imam SA. GSTPi-positive tumour microenvironment-associated fibroblasts are significantly associated with GSTPi-negative cancer cells in paired cases of primary invasive breast cancer and axillary lymph node metastases. Br J Cancer. 2011 Oct 11;105(8):1224-9. doi: 10.1038/bjc.2011.352. Epub 2011 Sep 6.