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Human Muscle PrimaCell™1: Normal Skeletal Muscle Cells

The Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells is for rapid (7-10 days) isolation and growth of normal Skeletal Muscle cells from human skeletal muscle tissues. This system features an optimal condition of tissue dissociation system, Muscle OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Muscle PrimaCell™ 1 System (Cat No. 2-96091):
Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Protocols
Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
Human Muscle OptiTDS™ 1: Tissue Dissociation System
Human Muscle FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Growth Medium
Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Mormeneo E, Jimenez-Mallebrera C, Palomer X, De Nigris V, Vázquez-Carrera M, Orozco A, Nascimento A, Colomer J, Lerín C, Gómez-Foix AM. PGC-1α induces mitochondrial and myokine transcriptional programs and lipid droplet and glycogen accumulation in cultured human skeletal muscle cells. PLoS One. 2012;7(1):e29985. Epub 2012 Jan 17.
[2] Danoviz ME, Yablonka-Reuveni Z. Skeletal muscle satellite cells: background and methods for isolation and analysis in a primary culture system. Methods Mol Biol. 2012;798:21-52.
[3] Guo X, Gonzalez M, Stancescu M, Vandenburgh HH, Hickman JJ. Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system. Biomaterials. 2011 Dec;32(36):9602-11. Epub 2011 Sep 23.

Cat No. Description Unit Price Order
2-96091 Human Muscle PrimaCell™1: Normal Skeletal Muscle Cells Kit $ 1228.50 
4-28091 Human Muscle OptiTDS™1: Tissue Dissociation System Kit $ 274.05 
7-15082 Human Muscle FibrOut™1: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 302.40 
9-46091 Human Muscle PrimaCell™1: Normal Skeletal Muscle Cells Growth Medium 5 x 100 ml $ 151.20 
9-47091 Human Muscle PrimaCell™1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80091 Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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