The Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells is for rapid (7-10 days) isolation and growth of normal Skeletal Muscle cells from human skeletal muscle tissues. This system features an optimal condition of tissue dissociation system, Muscle OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Human Muscle PrimaCell™ 1 System (Cat No. 2-96091):
Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Protocols
Human Muscle Tissue Preparation Buffer 1: Normal Skeletal Muscle Cells
Human Muscle OptiTDS™ 1: Tissue Dissociation System
Human Muscle FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Growth Medium
Human Muscle PrimaCell™ 1: Normal Skeletal Muscle Cells Growth Supplements with Serum (for 500 ml medium)
References:
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[2] Danoviz ME, Yablonka-Reuveni Z. Skeletal muscle satellite cells: background and methods for isolation and analysis in a primary culture system. Methods Mol Biol. 2012;798:21-52.
[3] Guo X, Gonzalez M, Stancescu M, Vandenburgh HH, Hickman JJ. Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system. Biomaterials. 2011 Dec;32(36):9602-11. Epub 2011 Sep 23.