The Human Placenta PrimaCell™: Normal Placenta Amniotic Cells is for rapid (7-10 days) isolation and growth of normal placenta amniotic cells from human placenta tissues. This system features an optimal condition of tissue dissociation system, Placenta OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.
Human Placenta PrimaCell™ System (Cat No. 2-96098):
Human Placenta PrimaCell™: Normal Placenta Amniotic Cells Protocols
Human Placenta Tissue Preparation Buffer: Normal Placenta Amniotic Cells
Human Placenta OptiTDS™: Tissue Dissociation System
Human Placenta FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Placenta PrimaCell™: Normal Placenta Amniotic Cells Growth Medium
Human Placenta PrimaCell™: Normal Placenta Amniotic Cells Growth Supplements with Serum (for 500 ml medium)
References:
[1] Sun K, Qu X, Gao L, Myatt L. Dexamethasone fails to inhibit the induction of cytosolic phospholipase A(2) expression by interleukin-1beta in cultured primary human amnion fibroblasts. Placenta. 2006 Feb-Mar;27(2-3):164-70.
[2] Ochsenbein-Kölble N, Bilic G, Hall H, Huch R, Zimmermann R. Inducing proliferation of human amnion epithelial and mesenchymal cells for prospective engineering of membrane repair. J Perinat Med. 2003;31(4):287-94.
[3] Carvalho AF, Santos JR, Gentz R, Bonjardim CA, Golgher RR, Ferreira PC, Kroon EG. Culture of human amniotic cells: a system to study interferon production. Placenta. 1998 May;19(4):307-14.