The Human Skin PrimaCell™ 1: Normal Epidermal Keratinocytes is for rapid (7-10 days) isolation and growth of normal epidermal keratinocytes from human skin tissues. This system features an optimal condition of tissue dissociation system, Skin OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Skin PrimaCell™ 1 System (Cat No. 2-96101):
Human Skin PrimaCell™ 1: Normal Epidermal Keratinocytes Protocols
Human Skin Tissue Preparation Buffer 1: Normal Epidermal Keratinocytes
Human Skin OptiTDS™ 1: Tissue Dissociation System
Human Skin FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Human Skin PrimaCell™ 1: Normal Epidermal Keratinocytes Growth Medium
Human Skin PrimaCell™ 1: Normal Epidermal Keratinocytes Growth Supplements with Serum (for 500 ml medium)

References:

[1] Le Gall-Ianotto C, Andres E, Hurtado SP, Pereira U, Misery L. Characterization of the first coculture between human primary keratinocytes and the dorsal root ganglion-derived neuronal cell line F-11. Neuroscience. 2012 May 17;210:47-57. Epub 2012 Mar 2.
[2] Vollmers A, Wallace L, Fullard N, Höher T, Alexander MD, Reichelt J. Two- and three-dimensional culture of keratinocyte stem and precursor cells derived from primary murine epidermal cultures. Stem Cell Rev. 2012 Jun;8(2):402-13.
[3] Sharma V, Singh SK, Anderson D, Tobin DJ, Dhawan A. Zinc oxide nanoparticle induced genotoxicity in primary human epidermal keratinocytes. J Nanosci Nanotechnol. 2011 May;11(5):3782-8.