The Human Skin PrimaCell™3 : Normal Skin Fibroblasts is for rapid (7-10 days) isolation and growth of normal skin fibroblasts from human skin tissues. This system features an optimal condition of tissue dissociation system, Skin OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Human Skin PrimaCell™3 System (Cat No. 2-96103):
Human Skin PrimaCell™3 : Normal Skin Fibroblasts Protocols
Human Skin Tissue Preparation Buffer 3 : Normal Skin Fibroblasts
Human Skin OptiTDS™3 : Tissue Dissociation System
Human Skin PrimaCell™3 : Normal Skin Fibroblasts Growth Medium
Human Skin PrimaCell™3 : Normal Skin Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Sandu C, Dumas M, Malan A, Sambakhe D, Marteau C, Nizard C, Schnebert S, Perrier E, Challet E, Pévet P, Felder-Schmittbuhl MP. Human skin keratinocytes, melanocytes, and fibroblasts contain distinct circadian clock machineries. Cell Mol Life Sci. 2012 May 25.
[2] Lee SH, Jin SY, Song JS, Seo KK, Cho KH. Paracrine effects of adipose-derived stem cells on keratinocytes and dermal fibroblasts. Ann Dermatol. 2012 May;24(2):136-43. Epub 2012 Apr 26.