The Human Vertebal PrimaCell™: Normal Intervertebral Disc Nucleus Pulposus is for rapid (7-10 days) isolation and growth of normal intervertebral disc nucleus pulposus from human vertebal tissues. This system features an optimal condition of tissue dissociation system, Vertebal OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Vertebal PrimaCell™ System (Cat No. 2-96114):
Human Vertebal PrimaCell™ : Normal Intervertebral Disc Nucleus Pulposus Protocols
Human Vertebal Tissue Preparation Buffer: Normal Intervertebral Disc Nucleus Pulposus
Human Vertebal OptiTDS™: Tissue Dissociation System
Human Vertebal FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Vertebal PrimaCell™: Normal Intervertebral Disc Nucleus Pulposus Growth Medium
Human Vertebal PrimaCell™: Normal Intervertebral Disc Nucleus Pulposus Growth Supplements with Serum (for 500 ml medium)

References:

[1] Huang B, Zhuang Y, Li CQ, Liu LT, Zhou Y. Regeneration of the intervertebral disc with nucleus pulposus cell-seeded collagen II/hyaluronan/chondroitin-6-sulfate tri-copolymer constructs in a rabbit disc degeneration model. Spine (Phila Pa 1976). 2011 Dec 15;36(26):2252-9.
[2] Murai K, Sakai D, Nakamura Y, Nakai T, Igarashi T, Seo N, Murakami T, Kobayashi E, Mochida J. Primary immune system responders to nucleus pulposus cells: evidence for immune response in disc herniation. Eur Cell Mater. 2010 Jan 14;19:13-21.