The Mouse Aorta PrimaCell™: Normal Aortic Endothelial Cells is for rapid (7-10 days) isolation and growth of normal mouse aortic endothelial cells from newborn or adult mouse aorta tissues. This system features an optimal condition of tissue dissociation system, aorta OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Aorta PrimaCell™ System (Cat No. 2-82004):
Mouse Aorta PrimaCell™ Normal Aortic Endothelial Cells Protocols
Mouse Aorta Tissue Preparation Buffer: Normal Aortic Endothelial Cells
Mouse Aorta OptiTDS™: Tissue Dissociation System
Mouse Aorta FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Aorta PrimaCell™: Normal Aortic Endothelial Cells Growth Medium
Mouse Aorta PrimaCell™: Normal Aortic Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Babu PV, Si H, Fu Z, Zhen W, Liu D. Genistein prevents hyperglycemia-induced monocyte adhesion to human aortic endothelial cells through preservation of the cAMP signaling pathway and ameliorates vascular inflammation in obese diabetic mice. J Nutr. 2012 Apr;142(4):724-30. Epub 2012 Mar 7.
[2] Liu F, Cao JG, Li C, Tan JS, Fu XH. Protective effects of 7-difluoromethyl-5,4'-dimethoxygenistein against human aorta endothelial injury caused by lysophosphatidyl choline. Mol Cell Biochem. 2012 Apr;363(1-2):147-55. Epub 2011 Dec 24.
[3] Tang R, Li Q, Lv L, Dai H, Zheng M, Ma K, Liu B. Angiotensin II mediates the high-glucose-induced endothelial-to-mesenchymal transition in human aortic endothelial cells Cardiovasc Diabetol. 2010 Jul 27;9:31.