The Mouse Brain PrimaCell™ 10: Normal Neuron Cells is for rapid (7-10 days) isolation and growth of normal mouse neuron cells from newborn or adult mouse brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Mouse Brain PrimaCell™ 10 System (Cat No. 2-82019):
Mouse Brain PrimaCell™ 10: Normal Neuron Cells Protocols
Mouse Brain Tissue Preparation Buffer 10: Normal Neuron Cells
Mouse Brain OptiTDS™ 10: Tissue Dissociation System
Mouse Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Brain PrimaCell™ 10: Normal Neuron Cells Growth Medium
Mouse Brain PrimaCell™10: Normal Neuron Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Molnár E. Long-term potentiation in cultured hippocampal neurons. Semin Cell Dev Biol. 2011 Jul;22(5):506-13. Epub 2011 Jul 22. Review.
[2] Kim HJ, Magrané J. Isolation and culture of neurons and astrocytes from the mouse brain cortex. Methods Mol Biol. 2011;793:63-75.
[3] Pacifici M, Peruzzi F. Isolation and culture of rat embryonic neural cells: a quick protocol. J Vis Exp. 2012 May 24;(63). pii: 3965. doi: 10.3791/3965.