The Mouse Brain PrimaCell™ 7: Normal Meningeal Cells is for rapid (7-10 days) isolation and growth of normal mouse meningeal cells from newborn or adult mouse brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

MouseBrain PrimaCell™ 7 System (Cat No. 2-82016):
Mouse Brain PrimaCell™ 7: Normal Meningeal Cells Protocols
Mouse Brain Tissue Preparation Buffer 7: Normal Meningeal Cells
Mouse Brain OptiTDS™ 7: Tissue Dissociation System
Mouse Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Mouse Brain PrimaCell™ 7: Normal Meningeal Cells Growth Medium
Mouse Brain PrimaCell™ 7: Normal Meningeal Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Brandenburg LO, Varoga D, Nicolaeva N, Leib SL, Podschun R, Wruck CJ, Wilms H, Lucius R, Pufe T. Expression and regulation of antimicrobial peptide rCRAMP after bacterial infection in primary rat meningeal cells. J Neuroimmunol. 2009 Dec 10;217(1-2):55-64. Epub 2009 Oct 30.
[2] Ibanez C, Ito D, Zawadzka M, Jeffery ND, Franklin RJ. Calponin is expressed by fibroblasts and meningeal cells but not olfactory ensheathing cells in the adult peripheral olfactory system. Glia. 2007 Jan 15;55(2):144-51.
[3] Ishiwata I, Ishiwata C, Iguchi M, Soma M, Sato Y, Sonobe M, Kiguchi K, Tachibana T, Ishikawa H. Biological characteristics of cultured cells derived from various types of human brain tumors. Hum Cell. 2004 Sep;17(3):117-24.